At 9:00am I turned off the chiller and took a sample of oysters from the overnight treatment. It appeared that many of the oysters from the overnight treatment were gaping slightly, but they still offered resistance during dissection. I dissected 6 cultch set oysters per population and 4 tile set oysters and put the whole body tissue in RNALater. I also dissected 10 oysters per population from the control treatment. Instead of moving the overnight oysters over to the control tank, I let the temperature gradually increase by draining some of the tank and adding ambient water. We switched the control tank over to a flow-through system with live algae dripping in. At 2:00pm I moved the overnight chilled oysters to the control tank. The hatchery folks are going to keep an eye on the oysters for 2 weeks, after which they’ll sample all of the survivors.

I took apart the tile trays and drained out the tank they had been in. Some tiles that had only a few oysters on them were not used in the temperature experiment. I dissected these and put the whole body in RNALater, figuring they could be used for genotyping or looking at epigenetic in the F2 generation. Some of them I took a picture for size data and some of them I measured with a ruler.

Samples from tiles:

1. SSB10
2. NFA7_1
3. NFA7_2
4. SSB11
5. NFA13R: 1.3cm x 1.4cm
6. HCA10_1: 1.3 x 1.2
7. HCA10_2: .9 x .7
8. HCA15 (top)
9. HCA6 (top)
10. NFB12 (middle)
11. NFB13R (2nd from top)
12. NFB7 (left)
13. HCA8