Tag Archives: Quibit

Monday 9/14/15

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Quantified broodstock samples that were extracted 8/19/15 and 9/11/15 with Qubit and ran out gel. Concentrations in ng/uL are below with score of quality based on gel.

9_14_15 Gel

  1. HC1_10: 27.7 ng/uL; 5
  2. NF1_8: 22.7 ng/uL; 3 (some degrad)
  3. HC1_9: 13.9; 3
  4. SS2_8: 16.8; 4.5
  5. HC1_8:22.8; 5
  6. NF1_2: 17.4; 4.5
  7. HC1_6: 23.5; 5
  8. HC1_1: 12.3; 4.5
  9. NF1_5: 18.1; 4.5
  10. SS2_7: 16.4; 5
  11. SS2_4: 10.2; 4
  12. SS2_3: 19.8; 5
  13. SS2_5: 7.83; 5
  14. HC1_5: 12.1; 5
  15. NF1_3: 27.8; 3 (some degrad)
  16. NF1_4: 8.9; 4
  17. HC1_4: 13.8; 4.5
  18. NF1_1: 9.13; 2.5 (low, some degrad)
  19. HC1_7: 14; 4.5
  20. NF1_7: 35.8; 5
  21. NF1_6: 16.1; 5
  22. HC1_2: 15.2; 2 (low)
  23. HC1_3: 43.5; 5
  24. SS2_6: 23.4; 5
  25. SS2_1; 5
  26. SS2_2; 1 (rerun)

Ethanol extraction of 10 broodstock DNA samples to prepare for test 2b-RAD library, following this protocol.

Population Sample Date extracted ng/uL Volume for 1 ug Volume sodium acetate Vol ethanol
Oyster Bay BS_2_3 19.8 50.50505051 5.05 113.64
Oyster Bay BS_2_4 10.2 98.03921569 9.80 220.59
Oyster Bay BS_2_5 7.83 127.7139208 12.77 287.36
Hood Canal BS_1_1 12.3 81.30081301 8.13 182.93
Hood Canal BS_1_2 15.2 65.78947368 6.58 148.03
Hood Canal BS_1_3 43.5 22.98850575 2.30 51.72
Hood Canal BS_1_4 13.8 72.46376812 7.25 163.04
Fidalgo BS_1_1 9.13 109.5290252 10.95 246.44
Fidalgo BS_1_2 17.4 57.47126437 5.75 129.31
Fidalgo BS_1_3 27.8 35.97122302 3.60 80.94

After drying samples for ~10 minutes at 37degC in a Centrivap Concentrator, I added 10 uL of nuclease-free water and left to rehydrate in 4degC fridge.