Common Garden Project
DNA extraction from Olympia oyster broodstock
Did not find NF2_8…
Olympia Oyster Population Structure Project
I started preparing genotype-by-sequencing libraries using the dried down samples from 10/21/15. Did an ApeKI digestion and ligation of adaptors.
|NEB Buffer 3||2.μL||100|
Added 20 uL of digestion master mix to each of the 48 wells. Incubated for 2 hours at 75degC then held at 4degC until ligation master mix was ready.
|T4 Ligase Buffer||5 μL||255|
|T4 Ligase||1.6 μL||81.6 μL|
Added 30 uL of ligation mastermix to each well to give 50 uL total. Incubated at 19degC for 1 hour and 65degC for 20 minutes. Cooled to 0degC and put in freezer.