Wednesday 9/23/15

Did more extractions of broodstock samples. I think once this test library is done the broodstock libraries should be 1st priority, with samples from the stressor experiment 2nd and other larvae samples 3rd.
- Used the EZNA kit with a 4 hour digestion time. All the tissue for HC1_16 was used up.

Finished the ethanol precipitation started on Tuesday 9/22/15. Left samples in 10 uL of water in 4degC overnight.
Qubit of samples for phylogeographic study. Made list of samples for GBS library.
- 1. BC1_12: 25.2
  2. BC1_8: 6.44 (8)
  3. BC2_7: 10.6
  4. BC2_13: 11.5
  5. BC2_9: 13 (8)
  6. BC3_13: 108
  7. BC3_12: 15 (8)
  8. BC4_2: 8.84
  9. BC4_17: 11.4 (8)
  10. CA1_18: 24.4
  11. CA1_1: 36
  12. CA1_2: 17.4 (8)
  13. CA2_9: 43.8
  14. CA2_12: 18.8 (5)
  15. CA3_6: 62.3 (5)
  16. CA4_1: 5.1
  17. CA4_7: 19.8 (8)
  18. CA5_10: 48.8 (8)
  19. CA6_8: 5.14
  20. CA6_15: 25.2 (7)
  21. CA7_11: 7.4
  22. CA7_16: 19.1
  23. CA7_15: 14.9 (8)
  24. OR1_ 1: 7.66
  25. OR1_5: 29.2 (7)
  26. OR2_6: 12.9
  27. OR2_12: 15.8
  28. OR2_15: 18.2 (7)
  29. OR3_15: 9.24
  30. OR3_20: 13.7 (7)
  31. WA10_16: 25.4
  32. WA10_13: 9.52 (8)
  33. WA11_10: 18.7
  34. WA11_22: 17.4
  35. WA11_4: 11.7
  36. WA11_17: 4.78 (8)
  37. WA12_15: 24.4
  38. WA12_11: 4.18 (8)
  39. WA13_12: 7.2
  40. WA13_5: 4.4
  41. WA13_15: 45.1 (9)
  42. WA9_2: 11.4 (7)
  43. WA1_16: 62 (8)
  44. Conch_1: 12.4
  45. conch_2: 12.3
  46. conch_4: 9.65
  47. CA5_12: 21.8 (repeat with 2)
  48. CA5_10 repeat within

1 thought on “Wednesday 9/23/15”

Getting My Genes Wet