3 thoughts on “2B-RAD Protocol

  1. Pingback: Friday 9/18/15 | Getting My Genes Wet

  2. Thank you for posting this information and making it public, it has been a huge help with planning my graduate project.

    That said, I was wondering if you remembered what type of sequencer you used for your 2b-RAD project? I’d assume an Illumina machine, but was it a Hiseq? Miseq? How many cycles did you run as well?

    Any advice would be greatly appreciated!


    • I’m so glad! I did about 70 samples per 50bp Illumina HiSeq 2500 lane. For PCR cycles I did a titration similar to that recommended in the Meyer’s protocol and settled on 17. Less is definitely more for cycles!


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