2B-RAD Protocol Posted on September 16, 2015 by ksil91 2b-RAD protocol Share this:TwitterFacebookLike this:Like Loading... Related
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Thank you for posting this information and making it public, it has been a huge help with planning my graduate project.
That said, I was wondering if you remembered what type of sequencer you used for your 2b-RAD project? I’d assume an Illumina machine, but was it a Hiseq? Miseq? How many cycles did you run as well?
Any advice would be greatly appreciated!
I’m so glad! I did about 70 samples per 50bp Illumina HiSeq 2500 lane. For PCR cycles I did a titration similar to that recommended in the Meyer’s protocol and settled on 17. Less is definitely more for cycles!