Wednesday 8/12/15

Larvae tanks

• NF_Tank2_160 (224) -> NF cultch set
• NF_Tank2_160 (100) -> swimmers only
• HC_Tank2_160 (224) -> HC cultch set
• HC_Tank2_160 (100) -> swimmers only
• SS_Tank2_160 (224) -> barely any, put back in tank
• SS_Tank2_160 (100) -> swimmers only

Tile Set

• Did live/dead counts for both A and B
• Did not add the unset larvae back to A
• Rinsed tiles

Cultch Set

• NF >450 -> NF >1000
• SS > 450 -> SS>1000
• SS_SetA -> SS > 450; SS_SetA

Thursday 8/13/15

Started dissections of tissue from the broodstock for subsequent DNA extractions with the help of Brent Vadopalas and the PSRF intern Ryann. First, we recorded the weight of each oyster in a family and placed it on a numbered pad.

Broodstock dissection setup

A picture was taken of the entire family with a ruler for later measurement in ImageJ. Effort was made to hold the phone level to avoid the impact of tilt on apparent size. We made a little assembly line, with Brent shucking before joining Ryann and I in dissecting out adductor muscle tissue and storing in 1.5 mL tubes with 1 – .75 mL RNALater. If there was not very much muscle tissue, mantle or the entire oyster were taken as well. Scalpels and forceps were rinsed sequentially in soapy water, bleach, and freshwater between each oyster. Fresh scalpels were used between populations. We got through SS2, SS1,SS3, SS4, SS5, and NF4, averaging about 45 minutes per family by the time we got the hang of it.

Husbandry

• Rinsed tiles and cultch
• Feeding

Friday 8/14/15

Larvae tanks

• NF_Tank2_160 (224) -> NF_New cultch set
• NF_Tank2_160 (100) -> dumped
• HC_Tank2_160 (224) -> none
• HC_Tank2_160 (100) -> dumped
• SS_Tank2_160 (all) -> dumped

Tile Set Counts

• NF_SetA
• SS_SetA
• HC_SetA
• HC_SetB
• SS_SetB

Dissections

Did NF2 and NF1 with a little help from Ryann on NF1