Monday 7/13/15

Today I decided to change what I was doing with the 100L larvae tanks. Instead of each population having one tank for new larvae and one for the “sick” larvae that survived the previous mortality, I will now have a tank for new larvae and a tank for larvae that are >160 microns. For the larvae in the “sick” tanks, I screened them over 160, 140, and 100 micron screens and kept those on the 160 in the cleaned out “160” tanks, kept the swimmers on 140 in the “new” tanks, and dumped out the 100s as they were mostly dead or sick. On Monday, Wednesday, and Friday I will now screen the larvae in the “new” tank to separate out all those that have grown to be 160 microns and will move those to the second “160” tank. I’ve been taking DNA samples of all newly spawned larvae and will now also take samples of those that reach 160 microns to see if there is significant loss of certain alleles as the larvae grow up.
Larval tank counts
  • HC_Tank2sick (160) -> HC_Tank2160
  • HC_Tank2sick (140) -> swimmers to HC_Tank1new
  • HC_Tank2sick (100) -> culled
  • HC_Tank1new (160) -> HC_Tank2160
  • HC_Tank1new (100) -> HC_Tank1new
  • NF_Tank1sick (160) -> NF_Tank2160
  • NF_Tank1sick (100) -> culled
  • NF_Tank2new (160) -> NF_Tank2160
  • NF_Tank2new (120) -> NF_Tank1new
  • NF_Tank2new (100) -> swimmers to NF_Tank1new
  • SS_Tank1new (160) -> none
  • SS_Tank1new (120) -> SS_Tank1new
  • SS_Tank1new (100) -> swimmers to SS_Tank1new
New Larvae
  • Lots: HC2,SS3,HC4,NF1,HC5
  • Some: SS1

Larvae Count Datasheets

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