PCR of 16S Region

• Verifying species identity as some may have actually been juvenile C. gigas

1. BC3_7 redo
2. BC3_11 redo
3. BC3_12
4. BC3_13
5. BC3_15
6. BC3_16
7. BC3_17
8. BC3_18
9. OR2_3
10. OR2_4
11. OR2_5
12. OR2_6
13. OR2_7
14. OR2_8
15. OR2_9
16. OR2_10
17. OR2_11
18. OR2_12
19. OR2_15
20. OR2_16
21. OR2_17
22. OR2_18
23. OR2_19
24. OR2_20
25. OR3_1
26. OR3_2
27. OR3_3
28. OR3_5
29. OR3_6
30. OR3_9
31. OR3_10
32. OR3_12
33. OR3_13
34. OR3_15
35. OR3_17
36. OR3_18
37. WA9_2
38. WA9_3
39. WA9_4
40. WA9_5
41. WA9_6
42. WA9_7
43. WA9_8
44. WA9_9
45. WA9_10
46. WA9_11

• CA2_1,3,4,5,7 (6/2)
• • redo all
• CA3_1,2,3,4,5,6
• • 5:3
    6:3
  • redo CA3_1,2,3,4
• WA12D_1,2,3,4,5,6
• • 1: 0
    2: 3
    3: 4
    4: 3
    5: 5
    6: 0
  • redo WA12D_1,6
• WA10T_1,2,4,5,6,7
• • 1: 5
    2:4.5
    4: 5
    5: 0
    6: 5
    7: 0
  • redo WA10T_4,6
• CA6_1,3,7,8,9 redos
• • 1: 2 (low, deg)
    3: 3(low)
    7: 3(low,deg)
    8: 5
    9: 0
• WA9_2,3,7 redos
• • 2: 2.5(low)
    3: 0
    7: 1
• OR1_1,2 redos
• • 1: 5
    2: 3.5(low)
• CA7_6 redos
• • 6: 3.5(low)
• BC3_12,13,15,16,17,18
• • 12: 5
    13: 4(bright, some smear)
    15: 2(low)
    16: 5
    17: 3.5(smear)
    18: 3.5(very very bright, smear)
• Note: Re-extract CA6_1,9, WA9_3,7, BC4