Ran out PCR from 11/4 and CA1_13-16

• Gel was not bright on left side
• All PCR bands were bright except 13,15,16
•  DNA extracts
  • 13: 5
  • 14: 5
  • 15: 3.5 (some degradation)
  • 16: 5

Extraction of CA5_5-16

• 56deg at 11:30a
• RNAse at 6:45p
• half were still gelatinous, added 1.5 uL more proteinase K and left for 1 hour