Wed. Nov. 5, 2014

Ran out PCR from 11/4 and CA1_13-16

  • Gel was not bright on left side
  • All PCR bands were bright except 13,15,16
  •  DNA extracts
    • 13: 5
    • 14: 5
    • 15: 3.5 (some degradation)
    • 16: 5

Extraction of CA5_5-16

  • 56deg at 11:30a
  • RNAse at 6:45p
  • half were still gelatinous, added 1.5 uL more proteinase K and left for 1 hour
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