Monday Nov. 17, 2014

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Run out test digestion on gel, 1 uL against 1 uL DNA
  • looked digested, but will run out 5uL of digested DNA for clearer gel pic
Purify Sequencing reactions
  • EtOH/EDTA precip of 86 samples following Pritzker protocol
  • Did not observe pellet in wells
  • After step 11, left in 65 deg oven for 5 minutes to ensure etOH evaporated, then wrapped in foil and left in 4deg C fridge

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