Seq Results (12/15/14)

Ostrea
  • BC3
    • 11
    • 12
    • 13
    • 17
    • 18
    • 15
  • OR2
    • 3
    • 4
    • 5
    • 6
    • 7
    • 8
    • 9
    • 10
    • 11
    • 12
    • 15
    • 16
    • 17
    • 18
    • 19
    • 20
  • OR3
    • 1
    • 2
    • 3
    • 5
    • 6
    • 9
    • 10
    • 12
    • 13
    • 15
    • 18
  • WA9
    • 2
    • 3
    • 6
    • 7
    • 5
    • 8
    • 9
    • 10
    • 11
  • b7_G4
  • b15_G8
  • b14_H7
  • b3_G2
  • b11_G6
  • b3_G2 (56% sequencing quality)
  • b32_H10(41)
Crassostrea
  • BC3_7
Did not work
  • BC3_16
  • OR3_17
  • WA9_4
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Sat. Dec. 13, 2014

Wanted to put sequences on sequencer-> still running
 (realized later that I actually can load sequencing plates while it’s running……)
Run out gel of BC4_4,5,6,7,8,9,10,11
  • 4: 5
  • 5: 3(low)
  • 6: 4.5
  • 7: 5
  • 8: 3(low, some deg)
  • 9: 4
  • 10: 2(very deg)
  • 11: 2(very deg)
Quibit
  • BC3
    • 1: 8.28
    • 2: 18.02
    • 3: 76.8
    • 4: 7.26
    • 9: 15.24
  • OR2
    • 1: 15.32
    • 2: 14.18
    • 13: 13.54
    • 14: 14.7
  • CA2
    • 3: 34.6
    • 4: 10.5
    • 5: 17
  • CA3
    • 3: 14
    • 4: 10.9
    • 5: 20
  • CA5
    • 8: 7.04

Tues. Dec. 9, 2014


1x         3x
Big Dye     1ul       3
Buffer        2 ul      6
Primerb       .66       1.98
Water        4.84      14.52
Do 1 and 2 ab
Add 1.5 ul of Sap/Exo product

3_1 Auto program
Quibit
  • CA6
    • 1: 5.92
      2: 10
      3: 3.84
      7: 7.36
      8: 5.14
      9: 4.84
      10: 14.5
      11: 7.01
      12: 11.2
  • CA5
    • 5: 18.3
      6: 19.5
      7: 16.3
      9: 15.5
      15: 26
      16: 48.6
  • CA4
    • 8: 57.4
      9: 8.1
      11: 6.64
      12: 6.70
      13: 16
      14: 12.8
  • CA7
    • 1: 9.2
      2: 17.2
      3: 4.56
      4: 11.3
      5: 6.8
      7: 7
  • CA1
    • 13: 3.04
      14: 6.3
      16: 7.26
      17: 84
    • 20: 10.7
    • 21: 21.4
    • 22: can’t find

Monday, Dec. 8, 2015

  • CA2
    • 1: 2.5 (low, deg)
      3: 4.5
      4: 4.5
      5: 5
      7: 4(some deg)
  • CA3
    • 1: 3.5(deg)
      2: 3(deg)
      3: 3.5(deg)
      4: 4
Quibit
  • BC2
    • 1: 8.66
      2: 6.62
      3: 7.44
      4: 4.82
      6: 7.52
      14: 10
  • BC1
    • 1: 11.7
      2: 10.5
      5: 13.9
      9: 10.9
      10: 7
      11: 5.6
  • WA1
    • 1: 19.4
      2: 79
      3: 10.5
  • WA13
    • 1: 7.1
      2: 13.1
      4: 6.1
      5: 4.4
      10: 9.44
      11: 6.7
      12: 7.2
  • OR1
    • 7: 17.1
      8: 26.1
      9: 19.9
      10: 13.5
      11: 23.8
      12: 29.2
Sap/Exo of PCR from 12/4
  • 2 uL Sap/Exo with 5 uL of PCR product