• Finished extraction from Nov 3.
• • RNAse 11:30-12:40
  • • used new RNAse halfway through, same conc
  • Eluted half in dH2O to see if difference with PCR
  • • CA1_1,2,14,18,20,17,13,12
    • BC3_5,8,3,6
  • CA1_17 <->CA1_15
• Ran out extractions on gel (BC3_01-9,11; CA1_1,2,4,5, 13-22)
• • BC3_1: 4
  • 2: 5
  • 3: 4 (some smear)
  • 4: 4
  • 5: 4
  • 6: 3.5
  • 7: 5
  • 8: 4.5
  • 9: 5
  • 11: 4.5
  • CA1_1: 4(very bright, some smear)
  • 2: 5
  • 3: 4
  • 4: 5
  • 13: 0
  • 14: 0
  • 15: 0
  • 16: 0
  • • Ladder not visible either, redo
  • 17: 5
  • 18: 4
  • 19: 4
  • 20: 4
  • 21: 5
  • 22: 5

• Setup PCR (36):
• • CA4_1
  • CA4_2
  • CA4_3
  • CA4_7
  • CA4_8
  • CA4_9
  • BC3_1
  • BC3_2
  • BC3_3
  • BC3_4
  • BC3_5
  • BC3_6
  • BC3_7
  • BC3_8
  • BC3_9
  • BC3_11
  • BC1_1
  • BC1_2
  • BC1_4
  • BC1_5
  • BC1_7
  • BC1_8
  • BC1_9
  • BC1_10
  • BC1_11
  • BC1_12
  • BC2_3
  • BC2_4
  • BC2_5
  • BC2_6
  • BC2_7
  • BC2_8

PCR protocol