Tues. Nov. 4, 2014

  • Finished extraction from Nov 3.
    • RNAse 11:30-12:40
      • used new RNAse halfway through, same conc
    • Eluted half in dH2O to see if difference with PCR
      • CA1_1,2,14,18,20,17,13,12
      • BC3_5,8,3,6
    • CA1_17 <->CA1_15
  • Ran out extractions on gel (BC3_01-9,11; CA1_1,2,4,5, 13-22)
    • BC3_1: 4
    • 2: 5
    • 3: 4 (some smear)
    • 4: 4
    • 5: 4
    • 6: 3.5
    • 7: 5
    • 8: 4.5
    • 9: 5
    • 11: 4.5
    • CA1_1: 4(very bright, some smear)
    • 2: 5
    • 3: 4
    • 4: 5
    • 13: 0
    • 14: 0
    • 15: 0
    • 16: 0
      • Ladder not visible either, redo
    • 17: 5
    • 18: 4
    • 19: 4
    • 20: 4
    • 21: 5
    • 22: 5
  • Setup PCR (36):
    • CA4_1
    • CA4_2
    • CA4_3
    • CA4_7
    • CA4_8
    • CA4_9
    • BC3_1
    • BC3_2
    • BC3_3
    • BC3_4
    • BC3_5
    • BC3_6
    • BC3_7
    • BC3_8
    • BC3_9
    • BC3_11
    • BC1_1
    • BC1_2
    • BC1_4
    • BC1_5
    • BC1_7
    • BC1_8
    • BC1_9
    • BC1_10
    • BC1_11
    • BC1_12
    • BC2_3
    • BC2_4
    • BC2_5
    • BC2_6
    • BC2_7
    • BC2_8

PCR protocol

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