Thurs. Sept. 11, 2014

DNA extractions

  • Set up 24 more samples for lysis
    • Humboldt Bay (best quality): CA6 7-12
    • N. Willapa (medium): WA1 7-12
    • Tomales Bay (poor): CA4 7-12
      • 10,11: ethanol, new tube, black/blue
    • Ladysmith (poor): BC4 16-21
  • Add 5 uL of 10 mg/mL RNase at 9:40pm
  • most tubes completely degraded
  • left until 10:40
  • CA6 11 required an extra 30 min of digestion and 2 uL more protienase A to fully digest
  • 1st centrifuge for 2 min instead of 1
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