Sunday, Sept. 7 2014

Sample storage
  • Finished checking all tubes to make sure tissue was covered in liquid at the bottom
    • 5 unlabeled tubes mixed between CA 5, 6, and WA O/P samples
  • replaced RNALater in BC pops with 80% ethanol
  • Ladysmith 10-17 and 18-25
    • frozen in blue capped tubes
    • 10-23
    • Stored in 80% ethanol
DNA extractions
  • Chose from 4 locations with variable storage quality
    • Humboldt Bay (best quality): CA6 1-6
    • N. Willapa (medium): WA1 1-6
    • Tomales Bay (poor): CA4 1-6
    • Ladysmith (poor): BC4 10-15
  • Left overnight (4:00pm) in 18.2 uL Proteinase K, mixing every 45 minutes
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