Sample storage
- Finished checking all tubes to make sure tissue was covered in liquid at the bottom
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- 5 unlabeled tubes mixed between CA 5, 6, and WA O/P samples
- replaced RNALater in BC pops with 80% ethanol
- Ladysmith 10-17 and 18-25
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- frozen in blue capped tubes
- 10-23
- Stored in 80% ethanol
DNA extractions
- Chose from 4 locations with variable storage quality
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- Humboldt Bay (best quality): CA6 1-6
- N. Willapa (medium): WA1 1-6
- Tomales Bay (poor): CA4 1-6
- Ladysmith (poor): BC4 10-15
- Left overnight (4:00pm) in 18.2 uL Proteinase K, mixing every 45 minutes
Getting My Genes Wet 